Fluorescein Conjugated Antiserum...

Description

Anti Human Complement C-5 (B1F) FITC, Low F/P - 2-4.9 - Direct Tag

• Monospecificity by solid phase adsorption for a strong tag with a low background
• High antibody titer equals more dilution and dollar value
• Choice of F/P ratio; high for indirect tags – low for direct tags
• FITC Conjugates
• Made in Goat
• IN VITRO Diagnostic Use

Description

        Antiserum to human C-5 is produced in goats by immunization with purified human C-5.  The gamma globulin fraction of the absorbed, monospecific antiserum is then prepared, followed by reaction with fluorescein isothiocyanate.  The excess dye is removed by dialysis and the conjugated globulin further fractionated on DEAE cellulose to remove under- and overlabelled protein.  Total protein is determined by the Biuret reaction and the concentration of fluorescein by comparison with a fluorescein dictate standard.  Sodium azide (0.1% w/v) is added as preservative.

C-5, the fifth component of complement reacts with the C_3b fragment of C₃ to form C_5a, and C_5b.^1,2.  The latter then reacts with C6 to initiate formation of the terminal active complex C_5-9.  C_5a, a small fragment of 8500 molecular weight, has potent anaphylotactic and chemotactic activity².

C-5 migrates as a B₁ globulin in serum and has a molecular weight of approximately 185,000.  Its average serum concentration is 7-8 mg/dl (70-80 mcg/ml).^3,4