Fluorescein Conjugated Antiserum...

Description

Anti Human Properdin FITC, Low F/P - 2-4.9 - Direct Tag

• Monospecificity by solid phase adsorption for a strong tag with a low background
• High antibody titer equals more dilution and dollar value
• Choice of F/P ratio; high for indirect tags – low for direct tags
• FITC Conjugates
• Made in Goat
• IN VITRO Diagnostic Use

Description

        Antiserum to human properdin is produced in goats by immunization with purified human properdin.  The gamma globulin fraction of the absorbed, monospecific antiserum is then prepared, followed by reaction with fluorescein isothiocyanate.  The excess dye is removed by dialysis and the conjugated globulin further fractionated on DEAE cellulose to remove under- and overlabelled protein.  Total protein is determined by the Biuret reaction and the concentration of fluorescein by comparison with a fluorescein diacetate standard.  Sodium azide (0.1% w/v) is added as preservative.

IgA antibodies, bacterial lipopolysaccharides, and some other carbohydrates cause activation of properdin and the complement system.  In some instances, indirect fluorescence with unlabelled antiserum (108002) to properdin may be necessary to detect small amounts of properdin.