Canine IgG Test Kit Radial...

Description

Canine IgG Test Kit Radial Immunodiffusion Test Kit

Radial Immunodiffusion Test for Quantitation of Canine IgG in Serum or Plasma.

1. Summary

Single radial immunodiffusion tests have evolved from the work of Fahey and McKelvey1 and Mancini et al2.  They are specific for the various proteins in serum or other fluids and depend on the reaction of each protein with its specific antibody.

When the wells in antibody containing gels are completely filled with the antigen, the precipitin rings which develop after 10-18 hours at room temperature are measured.  The diameter of the ring and the logarithm (base 10) of the protein concentration are related in a linear fashion.  Using appropriate reference standards, the concentration of unknown samples may be measured.

Immunoglobulin G (IgG) is one of the first line of defenses against encapsulated bacteria and streptococci.  The majority of the newborns IgG is obtained from the bitch's colostrum in the first 48 hours after birth providing the puppy nurses.  This is called passive transfer.  In passive transfer the IgG from colostrum provides antibodies to infectious agents that the bitch has been exposed to or immunized against.  The time it takes IgG to drop to half it's original titer in mammals ranges from 20 to 30 days.  The puppy can start producing it's own IgG in sufficient quantities after 30 to 80 days.