Bovine IgG Ultra Low Level...

Description

Bovine Ultra Low Level IgG Test Kit Radial Immunodiffusion Test Kit

*24 Well test kit that comes with 3 standards (high, medium and low) for 1-4 RID plates*

Radial Immunodiffusion Test for Quantitation of Bovine Ultra Low Level IgG in Serum or Plasma.

1. Intended Use

The quantification of bovine IgG in post suckle calves aids in the diagnosis of failure of passive transfer.

2. Summary

Single radial immunodiffusion tests have evolved from the work of Fahey and McKelvey1 and Mancini et al2.  They are specific for the various proteins in serum or other fluids and depend on the reaction of each protein with its specific antibody.

When the wells in antibody containing gels are completely filled with the antigen, the precipitin rings, which develop after 10-20 hours at room temperature, are measured.  The diameter of the ring and the logarithm (base 10) of the protein concentration are related in a linear fashion.  Using appropriate reference standards, the concentration of unknown samples may be measured.

Immunoglobulin G (IgG) is one of the first lines of defenses against encapsulated bacteria and streptococci.  The majority of the newborns IgG is obtained from the dam's colostrum in the first 16 hours after birth providing the calf nurses.  This is called passive transfer.  In passive transfer the IgG from colostrum provides antibodies to infectious agents that the dam has been exposed to or immunized against.  The time it takes IgG to drop to half it's original titer in mammals ranges from 20 to 30 days.  The calf can start producing it's own IgG in sufficient quantities after 30 to 80 days.