Description
Bovine Lactoferrin Test Kit Radial Immunodiffusion Test Kit
Radial Immunodiffusion Test for Quantitation of Bovine Lactoferrin in Serum, Whey and Colostrum.
- Intended Use
Bovine Lactoferrin is a non-heme iron binding glycoprotein found in milk and other secretory fluids and blood.
- Summary
Single radial immunodiffusion tests have evolved from the work of Fahey and McKelvey1 and Mancini et al2. They are specific for the various proteins in serum or other fluids and depend on the reaction of each protein with its specific antibody.
When the wells in antibody containing gels are completely filled with the antigen, the precipitin rings which develop after 10-20 hours at room temperature are measured. The diameter of the ring and the logarithm (base 10) of the protein concentration are related in a linear fashion. Using appropriate reference standards, the concentration of unknown samples may be measured.
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