Description
Sheep IgG Test Kit for Radial Immunodiffusion Test Kit
- Summary
Single radial immunodiffusion tests have evolved from the work of Fahey and McKelvey1 and Mancini et al2. They are specific for the various proteins in serum or other fluids and depend on the reaction of each protein with its specific antibody.
When the wells in antibody containing gels are completely filled with the antigen, the precipitin rings that develop after 10-20 hours at room temperature are measured. The diameter of the ring and the logarithm (base 10) of the protein concentration are related in a linear fashion. Using appropriate reference standards, the concentration of unknown samples may be measured.
Immunoglobulin G (IgG) is one of the first line of defenses against encapsulated bacteria and streptococci. The majority of the newborns IgG is obtained from the nanny's colostrum in the first 16 hours after birth providing the kid nurses. This is called passive transfer. In passive transfer the IgG from colostrum provides antibodies to infectious agents that the nanny has been exposed to or immunized against. The time it takes IgG to drop to half it's original titer in mammals ranges from 20 to 30 days. The kid can start producing it's own IgG in sufficient quantities after 30 to 90 days.
Sheep IgG RID document - PDF
Article Posted at Kent Laboratories about Sheep IgG RID:
Passive In-Vitro Tests In Zoo/Wild Animals
Pacific Northwest human immunodiagnostics laboratory Kent Laboratories, Inc. is a FDA registered facility situated between Vancouver, B.C. and Seattle, WA. One of the first commercial laboratories started in Washington state, they specialize in providing researchers and medical professionals with high tittered polyclonal antibodies at an economical price using antigens purified from normal serum.
Kent Laboratories produces antiserum which is solid phase adsorbed by affinity chromatography so that the final product has no soluble antigens known to sometimes interfere with sensitive testing procedures. The unique design of their RID (Radial Immunodiffusion) plates offers a simple, reliable and economical technique for the quantitation of human proteins in the clinical laboratory showing results between 18 and 48 hours.
The veterinary affiliate, Triple J Farms (registered with the USDA and NIH) is of special interest for in vitro testing in how their Sheep IgG RID Plate is cross reacting with the IgG of Giraffe, Rhinoceros, Bongo, Moose, Dall sheep, Bontebok, Elk and Mule Deer. Plans for more testing are in the works. Additionally, there is a need for building a standard from the specific species to give an accurate level of passive transfer. Blood will have to be collected from various animals before claims can be made but the preliminary findings are encouraging.
