Bovine IgM Test Kit Radial...

Description

Bovine IgM Test Kit Radial Immunodiffusion Test Kit

Radial Immunodiffusion Test for Quantitation of Bovine IgM in Serum or Plasma.

1. Intended Use

The quantification of Bovine IgM.

2. Summary

Single radial immunodiffusion tests have evolved from the work of Fahey and McKelvey1 and Mancini et al2.  They are specific for the various proteins in serum or other fluids and depend on the reaction of each protein with its specific antibody.

When the wells in antibody containing gels are completely filled with the antigen, the precipitin rings which develop after 10-20 hours at room temperature are measured.  The diameter of the ring and the logarithm (base 10) of the protein concentration are related in a linear fashion.  Using appropriate reference standards, the concentration of unknown samples may be measured.

Bovine IgM  occurs in serum, colostrum, and milk. IgM  is important in the primary immune response, complement fixation, and as an agglutinating antibody of the serum. IgM seems to be especially associated with parasitic infections of Anaplasma.The majority of the newborns IgM is obtained from the dam's colostrum in the first 16 hours after birth providing the calf nurses.  This is called passive transfer.  The calf can start producing it's own IgM in sufficient quantities after 30 to 80 days.